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. 2002 Oct 28;159(2):255–266. doi: 10.1083/jcb.200204023

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — PCM-1 localizes to cytoplasmic granules that show a dynamic distribution during the cell cycle. (A) immunoblots of HeLa cell extracts and Xenopus egg extracts probed with rabbit preimmune serum, immune serum against PCM-1, and the same immune serum after affinity purification. Positions of molecular mass markers are indicated on the left. (B and C) HeLa cell stained with antibodies against (B) PCM-1, and against (C) γ-tubulin. (D) Live image of a HeLa cell transfected with full-length PCM-1, tagged with GFP at the carboxy terminus. (E–H) Double immunofluorescence of PCM-1 (green) and tubulin (red), and staining of the DNA (blue) of HeLa cells (E) in interphase, (F) prophase, (G) metaphase, and (H) telophase. PCM-1 signal in E–H was photographed at identical exposure levels. Bars: (D, E, G, and H) 10 μm; same magnifications in B–D and F and G, respectively.