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. 2002 Oct 28;159(2):255–266. doi: 10.1083/jcb.200204023

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Microinjection of antibodies against PCM-1 causes aggregation of centrin and pericentrin. Xenopus A6 cells were microinjected with affinity-purified antibodies against PCM-1 (B, D–F, H, and J), or with control antibodies (A, C, G, and I). Cells were stained for immunofluorescence of (A and B) PCM-1, (C–F) centrin, (G and H) pericentrin, or (I and J) γ-tubulin. Insets show the same cells stained with Texas red–labeled anti–rabbit antibody to identify microinjected cells, and stained with DAPI to detect DNA (blue). Bars: (B and J) 10 μm; same magnifications in A and B and C–J, respectively.