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. 2002 Oct 28;159(2):255–266. doi: 10.1083/jcb.200204023

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Copyright © 2002, The Rockefeller University Press

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Figure 8. — Depletion of PCM-1, centrin-3, or ninein results in loss of microtubule anchoring at the centrosome. (A C, E, and G) U-2 OS cells were transfected with control dsRNA oligomers and stained in green for (A) PCM-1, (C) centrin-3, (E) ninein, and (G) pericentrin. Microtubules were stained in red, DNA in blue. (B, D, F, and H) Corresponding image pairs showing cells after treatment with siRNA against (B) PCM-1, (D) centrin-3, (F) ninein, (H) pericentrin. I, J, U-2 OS cells treated with (I) control oligomers or (J) centrin-3 siRNA. Red, centrin-3 immunofluorescence; green, ninein immunofluorescence. K, immunoblots of cells treated with control RNA or siRNA against (left) centrin-3, or (right) ninein, stained for centrin-3 or ninein, respectively. Bar in H, 10 μm.