Table I. Sensory axons of cGKI-deficient embryos reveal a longitudinal branching error within the DREZ.
| Embryo No. | Genotype | No. of injected DRGs | No. of DRGs with primarily rostral growth | No. of DRGs with equal bidirectional branching |
|---|---|---|---|---|
| E12 | ||||
| 498-10 | −/− | 3 | 3 | 0 |
| 498-11 | −/− | 4 | 4 | 0 |
| 517-1 | −/− | 1 | 1 | 0 |
| 519-2 | −/− | 2 | 2 | 0 |
| 519-9 | −/− | 3 | 0 | 1 |
| 520-1 | −/− | 1 | 0 | 1 |
| 520-7 | −/− | 2 | 2 | 0 |
| 522-10 | −/− | 2 | 2 | 0 |
| 524-5 | −/− | 1 | 1 | 0 |
| 524-8 | −/− | 1 | 0 | 1 |
| 527-6 | −/− | 2 | 0 | 2 |
| 528-1 | −/− | 1 | 0 | 1 |
| 498-1 | +/+ | 2 | 0 | 2 |
| 498-2 | +/+ | 4 | 0 | 4 |
| 498-6 | +/+ | 2 | 0 | 2 |
| 498-9 | +/+ | 3 | 0 | 3 |
| 517-8 | +/+ | 1 | 0 | 1 |
| 519-1 | +/+ | 1 | 0 | 1 |
| 519-4 | +/+ | 2 | 0 | 2 |
| 522-7 | +/+ | 2 | 0 | 2 |
| 522-8 | +/+ | 1 | 0 | 1 |
| 524-4 | +/+ | 3 | 0 | 3 |
| 524-6 | +/+ | 2 | 0 | 2 |
| 524-7 | +/+ | 1 | 0 | 1 |
| E13 | ||||
| 483-1 | −/− | 1 | 1 | 0 |
| 483-4 | +/− | 2 | 1 | 1 |
| 483-5 | +/− | 2 | 0 | 2 |
Number of DRGs labeled by DiI is given. The intensity of rostral and caudal segments within the spinal cord was measured in identical fields using NIH image software. The ratio of rostral/caudal staining varied from 1:0.51 to 1:0.85 in labeled DRGs with a primarily rostral growth in the absence of cGKI (mean value, 1:0.66 ± 0.11). All others were considered as equal bidirectional growth. In wild-type DRGs, we observed a 1:1.02 ratio (mean).