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. 2002 Nov 11;159(3):465–476. doi: 10.1083/jcb.200205115

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Antibodies against the NH₂ terminus of p120 detect extremely low levels of p120 in SW48 cells. (A) p120 localization with NH₂-terminally directed p120 mAbs. SW48 cells were costained with p120 mAb's 5A7 or 6H11 (a and c), and E-cadherin mAb C-20820 (b and d). (B) Quantitative analysis of p120 expression was performed by immunoprecipitating p120 from RIPA cell lysates with either NH₂-terminally directed mAbs 5A7 or 6H11, or the COOH-terminally directed mAb pp120 (top). The immunoprecipitates were split and Western blotted with mAb 5A7 (lanes 1–6) or mAb pp120 (lanes 7–12). HCA7 cells expressing normal levels of p120 were used for comparison. The blots are overexposed to visualize the extreme low levels of p120.

Figure 3. — Antibodies against the NH₂ terminus of p120 detect extremely low levels of p120 in SW48 cells. (A) p120 localization with NH₂-terminally directed p120 mAbs. SW48 cells were costained with p120 mAb's 5A7 or 6H11 (a and c), and E-cadherin mAb C-20820 (b and d). (B) Quantitative analysis of p120 expression was performed by immunoprecipitating p120 from RIPA cell lysates with either NH₂-terminally directed mAbs 5A7 or 6H11, or the COOH-terminally directed mAb pp120 (top). The immunoprecipitates were split and Western blotted with mAb 5A7 (lanes 1–6) or mAb pp120 (lanes 7–12). HCA7 cells expressing normal levels of p120 were used for comparison. The blots are overexposed to visualize the extreme low levels of p120.