Figure 5.

Inhibition of dynein motor activity results in peripheral localization of HIV particles. (A) Cells were microinjected with antidynein IC74 mAb (1.5 mg/ml) and rhodamine-dextran marker (cell outlined in blue; white outlines are uninjected cells), infected for 1 h with DiD (red)-labeled GFP–Vpr-labeled HIV (green) at 37°C, and then fixed. (B and C) Magnification of boxed regions. Arrows show GFP-positive, DiD-negative particles in the perinuclear region of the uninjected cell (B) and peripheral region of the injected cell (C). (D) Quantification of DiD-negative particle progression toward the nucleus. Relative migration is calculated as described in Fig. 3. The graph represents the majority of particles in uninjected (none) and injected (IC74) cells from A. Bars, 10 μm.