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. 2002 Nov 11;159(3):441–452. doi: 10.1083/jcb.200203150

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Copyright © 2002, The Rockefeller University Press

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Figure 8. — Some HIV reverse transcription complexes contain p24^CA protein. Hos cells that express CD4 and CCR5 were injected with Alexa–dUTP (red) and infected with GFP–Vpr-labeled YU strain of HIV (green) (MOI ∼10) for 4 h, fixed, and stained with p24^CA antibody. (A) Perinuclear region of a cell with some nuclear incorporation of Alexa–dUTP. (B) Merged (left) and individual color images of a p24^CA-negative RTC. Note the presence (top right) of a dUTP-negative, p24^CA-positive particle. (C) Merged and individual images of p24^CA-positive RTCs. (D) p24^CA-positive and -negative RTCs associate with microtubules. Microtubules (blue) were stained in addition to p24^CA, and images were collected in four color spectra. (E) Shows the same two RTCs without microtubules, and (F) shows the p24^CA (blue) staining profile. Bars: (A) 5 μm; (B–F) 2 μm.