Figure 2.

Combined detection of SRP RNA and nucleolar fibrillar centers. NRK cells were transfected with a GFP fusion of the RNA polymerase I–specific transcription factor UBF and, in parallel, untransfected NRK cells were immunostained for UBF. Both sets of cells were then subjected to in situ hybridization for SRP RNA with PNA probes (Materials and methods). (A–F) SRP RNA and UBF immunostaining. (A) Immunostained UBF. (B) SRP RNA. (C) Merged. (D–F) Nearest-neighbor deconvolutions of A–C, respectively. (G–L) SRP RNA and GFP-UBF. (G) GFP-UBF. (H) SRP RNA. (I) Merged. (J–L) Nearest-neighbor deconvolutions of G–I, respectively. (C, F, I, and L, insets) Nucleolus at higher magnification.