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. 2002 Nov 25;159(4):625–635. doi: 10.1083/jcb.200208154

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — Predocked lysosomes are primarily responsible for calcium induced exocytosis. Cells loaded with fluorescent dextran were treated with 10 μM calcium-ionophore, 0.2 U/ml thrombin, or 20 nM bombesin. The cells were continuously imaged using TIR-FM. A frame-by-frame analysis was performed to identify the exocytosing and motile lysosomes. In the case of both (A) CHO cells and (B) murine embryonic primary fibroblasts, treatment with calcium ionophore, thrombin and bombesin led to lysosomal exocytosis. The murine embryonic primary fibroblasts showed approximately fivefold greater exocytosis than the CHO cells. (C) Increase in calcium caused both by ionophore and IP3 agonists, bombesin and thrombin led to a reduced motility of the lysosomes in the evanescent field. (Lysosomes that appeared or disappeared from the evanescent field, or moved >1 μm within the evanescent field were classified as motile.)