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. 1998 Sep 29;95(20):11863–11868. doi: 10.1073/pnas.95.20.11863

Figure 1.

Figure 1

(A) Map of the transgene. The PEBP2βMYH11 cDNA inserted into the BglII site of the human MRP8 promoter cassette corresponded to the most common inv(16) breakpoint. The nucleotide locations of the breakpoint within the coding sequences of PEBP2β and MYH11 are shown. White boxes represent noncoding portions of MRP8 exons. Solid lines represent 5′ and 3′ flanking sequences and intron 1 of MRP8. (B) Expression of the transgene. Whole-cell lysates were subjected to denaturing PAGE and Western blotting using a rabbit polyclonal anti-Pebp2β antiserum. The signal at 70 kDa corresponds to the Pebp2βSmmhc protein. The first two lanes are lysates of NIH 3T3 cells that contained retroviral vectors expressing either a lacZ or a PEBP2βMYH11 cDNA. The remaining lanes are lysates of tissues from control FVB/N mice or transgenic mice of lines Tg259 or Tg237. (C–F) Immunofluorescence of bone marrow neutrophilic cells. (C and D) Control. (E and F) Tg237. (C and E) Anti-Pebp2β antiserum. (D and F) Hoechst 33258. (Original magnification, ×250.)