Figure 4.
Morphology of bone marrow cells cultured in vitro. Bone marrow cells were grown in semisolid methylcellulose medium in the presence of G-CSF at 2.5 × 105 cells/ml for 7 days, and cytospins of harvested cells were prepared. Numerous macrophages (arrow) and neutrophilic cells (arrowhead) were observed in the control samples. Neutrophilic cells were decreased in the samples from transgenic mice. (Insets) Neutrophil morphology. Neutrophils derived in vitro from the bone marrows of transgenic mice exhibited atypical morphology. (Original magnification, ×250.)