Table I. Percentage of transfected COS7 cells displaying various Golgi morphologies after etoposide treatment for 24 h.
Transfected construct | Tight and reticular | Loose and reticular | Dispersed and punctate |
---|---|---|---|
% | % | % | |
Wild-type p115 | 18.4 | 42.9 | 38.7 |
TEKD757Ala p115 | 24.0 | 58.3 | 17.3 |
Results are the averages of duplicate samples from two separate experiments. In each case, 1,400 cells were counted by two independent observers. The expression levels of the wild-type and TEKD757Ala p115 were identical, as determined by Western blot analysis (unpublished data).