Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Nov 25;159(4):549–555. doi: 10.1083/jcb.200208159

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — hNuf2-depleted kinetochores retain the spindle checkpoint proteins Mad2 and BubR1 and the motor proteins CENP-E and cytoplasmic dynein. Cells were mock transfected (A) or transfected with an hNuf2 siRNA (B) and fixed for immunofluorescence 48 h after transfection. The cells were stained using the indicated antibodies (left columns) and a kinetochore marker (CREST serum; center columns). Each image shows one kinetochore pair. (C) Kinetochore localization of CENP-E and dynein in nocodazole-treated cells. Cells were subjected to nocodazole treatment and immunofluorescence analysis.