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. 2002 Jul 22;158(2):283–292. doi: 10.1083/jcb.200112025

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — CICR triggered by apical-localized uncaging is inhibited by pharmacological inhibition of IP₃Rs and RyRs. Traces show normalized fluorescence from regions of interest, shown in accompanying transmitted images, during representative continuous experiments on cells loaded with calcium-sensitive dye and NP-EGTA. (A) The IP₃ receptor antagonist caffeine blocks CICR induced by localized uncaging. (a) Application of 20 mM caffeine completely inhibits Ca²⁺ release triggered by 50 nM ACh, confirming the block of IP₃Rs. (b) Later, still in the presence of caffeine, apically localized uncaging events (area shown by orange circle superimposed on fluorescence image) fail to trigger any CICR response. (B) The RyR antagonist ryanodine blocks CICR induced by localized uncaging. (a) 50 μM ryanodine completely blocks calcium elevations caused by 25 nM ACh. (b) In the continuous presence of ryanodine, multiple localized uncaging events (targeted at orange circle superimposed on fluorescence image) consistently fail to trigger CICR. F_pp ratio = 2.08.