Table I. Morphometric analysis of dynamic p150Glued microtubule binding.
Construct | Rate of growth | Length of microtubule labeling |
---|---|---|
μm/s | μm | |
p150Glued | ||
Full-length | 0.30 ± 0.03 | 1.57 ± 0.35 |
aa 1–330 | 0.23 ± 0.02 | 1.25 ± 0.24 |
Acetate | 0.19 ± 0.04 | 1.06 ± 0.20 |
Temperature shift | 0.15 ± 0.02 | ND |
Image stacks were imported into Metamorph for calibrated measurement of dynactin labeling and rates of microtubule growth.