Figure 1.

N-cadherin and β-catenin accumulation in cell–cell junctions is dependent on calcium and N-cadherin activity. C2C12 cells were left untreated (a, f, and k–m) or treated with 2 mM EGTA for 60 min (b, g, and n–p). The EGTA-containing medium was then replaced with calcium containing medium alone for 60 min (c, h, q, r, and s) or in the presence of anti–N-cadherin antibodies (d, i, and t–v). Alternatively, anti–N-cadherin antibody was added for 20 h during cell–cell contact establishment (e, j, w, x, and y). Enlarged views of the cell–cell contact sites (k–y). Cells were stained for N-cadherin (a–e, k, n, q, t, and w) or for β-catenin (f–j, l, o, r, u, and x). Dotted lines represent the cell–cell contact region. Bars, 10 μm.