Table I. Quantitative analysis of the cyclin-dependent kinase inhibitors p21 and p27 and muscle-specific proteins expression and myotubes formation in control and anti–N-cadherin antibodies-treated cells.

	% of p21 expression	% of p27 expression	% of myogenin		% of troponin T		% of myotubes
	D4	D4	D3	D5	D3	D5	D3	D5	D7
Control cells (preimmune serum)	100	100	68	89	52	85	21	53	61
Anti–N-cadherin antibody (#1)	ND	ND	1	3	2	5	0	2	6
Anti–N-cadherin antibody (#2)	20	25	2	3	1	2	0	3	5
Anti–N-cadherin antibody (#3)	18	21	0	1	0	2	0	0	0

Results are means of several experiments (n = 5) and are the percentage of muscle-specific proteins expression and myotubes formation. All SEMs were <5%. C2 myoblasts were treated with preimmune serum or anti–N-cadherin antibodies (three differents antisera were used #1, #2, and #3), then placed in differentiation medium, fixed, and analyzed for myogenin and troponin T expression and myotube formation at the indicated times. For p21 and p27 detection, equal amount of protein extract from C2C12 myoblasts treated as described above were analyzed by immunoblotting and quantified as described in Materials and methods. D, time in culture in days.