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. 2002 Sep 2;158(5):873–884. doi: 10.1083/jcb.200202032

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — DmEB1 is localized to the plus ends of microtubules in S2 cells. (a) Immunoblot of Dm EB1 in extracts prepared from Drosophila embryos (E) (0–4 h old) and Schneider cells (S). Molecular masses (in kD) are provided to the left. (b) Quantitative immunoblotting for Dm EB1 protein in cultures subjected to RNAi for 0–6 d for EB1 (top) and GFP (bottom). All lanes contained an equal load of protein as determined by blotting for tubulin (not depicted). (c–h) Immunolocalization of Dm EB1 (green), microtubules (red), and DNA (blue) in Drosophila S2 cells. (c) Cells plated on poly-l-lysine before fixation and staining. (d) A cell plated on concanavalin A and allowed to spread for 2 h before fixation shows considerable improvement in imaging of the microtubule cytoskeleton. The magnification in panels c and d are the same. Bar, 5 μm. S2 cells in prophase (e), metaphase (f), anaphase (g), and telophase (h). Bar, 5 μm. Immunofluorescent localization of Dm EB1 (green), tubulin (red), and DNA (blue) in Drosophila synctitial blastoderm-stage embryos during anaphase (i) and telophase (j). Dm EB1 shows a marked accumulation at spindle poles and on the midbody. Bar, 10 μm.