Table III. Quantitation of chromosome segregation defects induced by injection of Dm EB1 antibodies into cycle 13 Drosophila embryos.

Nuclear phenotype	Control		Anti-EB1
	Proximal	Distal	Proximal	Distal
% Normal (n)	90.4 (57)	98.3 (60)	8.6 (14)	79.0 (111)
% Lagging (n)	9.5 (4)	1.6 (1)	30.0 (49)	18.0 (26)
% Bilobed (n)	0	0	8.6 (14)	0.7 (1)
% Stretched (n)	3.2 (2)	0	31.0 (51)	2.2 (3)
% No segregation (n)	0	0	21.8 (34)	0

The defects are expressed as percentages with the number of spindles observed provided in parentheses. Spindle defects were quantitated both proximal and distal to the site of microinjection in buffer- and antibody-injected embryos. Individual spindles were scored as “normal” if their mitosis occurred unperturbed by the injections, “lagging” if one or more of the chromosomes failed to segregate at anaphase, “bilobed” if the decondensed daughter nuclei were distinct at the end of mitosis but remained connected by a bridge of chromatin, or “stretched” if the sister chromosomes exhibited a partial segregation and decondensed to an elongated mass between the two poles.