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. 1998 Sep 29;95(20):11869–11874. doi: 10.1073/pnas.95.20.11869

Figure 1.

Figure 1

Targeting strategy for SP-D gene disruption and confirmation of the expected null mutation by genotype analysis. (a) The thin line depicts the mouse SP-D gene, with the eight exons indicated by boxes. Pgk-neo replaced all of exon 2 that contains the translation start site for SP-D. The 600-bp probe used for Southern blot screening is located 5′ to the targeting vector. B, BstXI; r, EcoRI; H, HindIII; X, XbaI. (b) Southern blot of tail genomic DNA from wild type (+/+), heterozygous (+/−), and SP-D null (−/−) mice and embryonic stem clone DNA after BstXI digestion. The 6.2-kb BstXI band is the normal allele and the 3.1-kb BstXI band the mutant allele.