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Analysis of SP-D mRNA (a) and protein in the lungs (b) and BAL (c) of null mice. (a) Northern blot of lung RNA demonstrating the absence of a 1.35-kb full-length mRNA for SP-D in the lungs of −/− mice. The low level 1-kb mRNA lacking exon 2 is visible. (b) Western blot of lung tissue demonstrating the absence of SP-D in the −/− lung. (c) Western blot of BAL demonstrating the absence of SP-D in −/− BAL.
