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. 2002 Sep 16;158(6):1067–1078. doi: 10.1083/jcb.200204063

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — β-cat– and TCF4–dependent transcription of the HIS3 reporter gene allows for yeast cell growth on medium lacking histidine. His⁻ yeast containing β-cat and TCF4 plasmids with WT or Mut 5X LEF HIS3 reporter plasmids were grown on media lacking histidine with either glucose or galactose. (A) Photograph of yeast growth after 4 d. Yeast grown on glucose do not express β-cat or TCF4 and therefore cannot grow on medium lacking histidine (left). Yeast cells grown on galactose are induced to express β-cat and TCF4. Cells containing a WT 5X LEF HIS3 reporter are able to grow, whereas those containing a Mut 5X LEF HIS3 reporter cannot (right). (B) Western blot demonstrating expression of TCF4 and β-cat proteins under induced (lane 2), but not uninduced (lane 1), conditions. Blots were probed with antibodies directed against the FLAG epitope (top) and against β-cat (bottom).

Figure 3. — β-cat– and TCF4–dependent transcription of the HIS3 reporter gene allows for yeast cell growth on medium lacking histidine. His⁻ yeast containing β-cat and TCF4 plasmids with WT or Mut 5X LEF HIS3 reporter plasmids were grown on media lacking histidine with either glucose or galactose. (A) Photograph of yeast growth after 4 d. Yeast grown on glucose do not express β-cat or TCF4 and therefore cannot grow on medium lacking histidine (left). Yeast cells grown on galactose are induced to express β-cat and TCF4. Cells containing a WT 5X LEF HIS3 reporter are able to grow, whereas those containing a Mut 5X LEF HIS3 reporter cannot (right). (B) Western blot demonstrating expression of TCF4 and β-cat proteins under induced (lane 2), but not uninduced (lane 1), conditions. Blots were probed with antibodies directed against the FLAG epitope (top) and against β-cat (bottom).