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. 2002 Sep 16;158(6):1039–1049. doi: 10.1083/jcb.200203123

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Copyright © 2002, The Rockefeller University Press

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Figure 9. — Fumonisin B1 prevents ceramide elevation induced by S1P in mSPP-1 transfectants. Vector- or mSPP-1–transfected HEK 293 cells were treated with 5 μM S1P or dihydro-S1P in the absence or presence of 25 μM fumonisin B1 for 48 h as indicated. Ceramide (A) and sphingosine levels (B) were determined as described in the Materials and methods. Sphingosine levels in vector and mSPP-1 transfectants treated with vehicle, S1P, or dihydro-S1P were 0.1 ± 0.02 and 0.2 ± 0.02, 0.9 ± 0.3 and 1.1 ± 0.3, 1 ± 0.4 and 1 ± 0.4 pmol/nmol phospholipid, respectively. Data are the means ± SEM of three independent experiments, each performed in duplicate. (Inset) mSPP-1–transfected HEK 293 cells were treated with vehicle, S1P (5 μM), or dihydro-S1P (5 μM) in the absence (−) or presence (+) of 25 μM fumonisin B1 for 48 h. Percentages of apoptotic cells were determined by Hoechst staining. A minimum of 500 cells in each field was scored.