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. 2002 Sep 16;158(6):1079–1087. doi: 10.1083/jcb.200202049

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — MITF can rescue growth suppression of dnTCF in melanoma cells. (A) Colony-forming assays in two human melanoma cell lines (501mel and SK-MEL-5) show rescue of clonogenic survival in the presence of dnTCF by wild-type MITF, whereas c-Myc is further growth inhibitory (B) Clonogenic survival in B16 melanoma demonstrates suppression by dnTCF and rescue by wild-type MITF, but not c-Myc (quantitative results are normalized to vector control). Experiment done in triplicate and quantitated results are graphed. Indicated with * is significant with P < 0.03 and ** significant with P < 0.01 as statistically calculated using Student's t test for unpaired samples. (C) Control colony formation assays in B16 cells showing growth suppressive effects of dnTCF and c-Myc in relation to MITF and vector control. (D) MITF rescues apoptosis induced by dnTCF but cannot relieve cell cycle inhibition in B16 melanoma cells. In contrast c-Myc bypasses dnTCF antiproliferative effects (S-phase repression), but significantly increases the apoptotic population. Apoptosis is measured as sub-G1 peak from propidium iodide staining. The quantitation was performed using ModFit v2.0 and represents values normalized to vector control as baseline. Averages from four independent experiments are presented (including error bars for standard deviation of mean). (E) Soft agar assay showing a representative example of macroscopic colonies of SK-MEL-5 cells 20 d after infection with the indicated retroviruses.