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. 2002 Sep 16;158(6):1089–1096. doi: 10.1083/jcb.200203091

Figure 3.

Figure 3.

Representative examples of [Ca2 + ]i measurements in C57 muscle fibers. Fibers were incubated for 5 min in potassium aspartate (10 mM EGTA) solution in the presence or absence of 1 μM thapsigargin. Next, they were repolarized for 5 min in a Krebs solution (50 μM EGTA). Finally, depolarization in potassium aspartate solution induced a [Ca2+]i transient that was taken as an index of the amount of releasable Ca.