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. 2002 Sep 30;158(7):1277–1285. doi: 10.1083/jcb.200208083

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Hsp70 binding to CD40 is mediated by the NH₂-terminal ATPase domain and is competed by Hip. (A) Human His6-tagged Hsp70, its NH₂- or COOH-terminal domains, or recombinant bacterial DnaK was incubated either with GST-CD40 or with GST. (B) Recombinant DnaK was incubated with ADP, ATP, or an excess of peptide C, followed by addition of GST-CD40 or GST alone. (C) His6-tagged N70 was incubated in the presence of ADP or ATP, followed by incubation with a 10-fold molar excess of Hsp70 in the presence of ADP or ATP. (D) Recombinant human His6-tagged Hsp70 protein was incubated with a fivefold molar excess of either recombinant Hip protein or Bag-1, and with GST-CD40 or GST as a control. Bound protein was analyzed after affinity purification on glutathione-sepharose by immunoblotting with an antibody directed against the NH₂-terminal His6 tags, or with an antibody directed against DnaK.