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. 2002 Apr 1;157(1):125–136. doi: 10.1083/jcb.200108089

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — MURF-1 is detected in the M-line region of the sarcomere, diffuse in the cytoplasm, and in nuclei in fetal rat cardiac myocytes. Rat cardiac myocytes were labeled with polyclonal anti–MURF-1 antibodies followed by Texas red–conjugated secondary antibodies (a, c, and e), and with monoclonal α-actinin antibodies followed by Cy2-conjugated secondary antibodies (b and d). DAPI stain was added to identify nuclei (f). Note, inset in b is a merged image of MURF-1 (red) and α-actinin (green) staining. MURF-1 staining in the M-line region (a, double arrows), diffuse (c and e, arrowheads), and/or in nuclei with varying staining intensities (a and e). N, nucleus. Bars, 10 μm.