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. 2002 Apr 1;157(1):125–136. doi: 10.1083/jcb.200108089

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Expression of GFP–MURF-1 does not appear to affect the integrity of thin filament or Z-line components. Myocytes expressing GFP–MURF-1 (c) or GFP alone (a) were stained for Z-lines with saromeric α-actinin antibodies (b and d) and show regular, striated staining. Triple-labeling studies in GFP–MURF-1–transfected cells (h), using Texas red–conjugated phalloidin (j) and antibodies to titin A168–170 (i), determined that thin filament integrity is not affected upon disruption of COOH-terminal titin in identical myofibrils. GFP-transfected cells (e) exhibited normal actin filament (g) and COOH-terminal titin (f) staining. Double arrows mark regular, striated staining. Single arrows mark disrupted titin staining. Bars, 10 μm.