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. 2002 Apr 1;157(1):125–136. doi: 10.1083/jcb.200108089

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Copyright © 2002, The Rockefeller University Press

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Figure 8. — The central portion of MURF-1 is involved in maintaining the structure of COOH-terminal titin. Myocytes expressing defined regions of MURF-1 were stained with anti-titin A168–170 antibodies. Expression of the RING–GFP (a and b) or Tail–GFP (c and d) proteins did not appear to affect the integrity of COOH-terminal titin. Expression of GFP fusion proteins of the central MURF region (Central; i and j), the RING plus central regions (Tailless; g and h), and the central plus tail regions (RINGless; e and f) severely disrupted titin's COOH-terminal region (single arrow marks disrupted titin A168–170 staining; double arrows mark regular, striated titin A168–170 staining). Note that titan staining colocalized with many of the GFP–MURF-1 aggregates (g–j, arrowheads). Bar, 10 μm.