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. 2002 Apr 1;157(1):45–62. doi: 10.1083/jcb.200112127

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — The SNARE motif-related region of p115 inhibits NSF-catalyzed Golgi reassembly. (A) MGFs were incubated at 37°C for 1 h with NSF, SNAPs, and p115 (130 nM) in the presence or absence of 13 μM of the indicated SNARE or peptide. CC2-4 peptides were also added in combination. CC1-4 were also preincubated with an equimolar amount of His–GOS-28 or His–syntaxin-5 and then added. Reactions were terminated by fixation, processed for EM, and the amount of relative cisternal regrowth was determined. 100% relative cisternal regrowth represents an increase from 25 to 75% of the total membrane present as cisternae. Values represent means ± SEM (n = 3–6). (Inset) Increasing amounts of CC1 were added to the NSF reaction. Values represent means ± SEM (n = 3). (B) MGFs were incubated at 37°C for 1 h with NSF, SNAPs, and p115 with or without various anti-SNARE antibodies. Reactions were processed as in A. Values represent means ± SEM (n = 3).