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. 2002 Mar 4;156(5):791–795. doi: 10.1083/jcb.200111068

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — AAK1 copurifies with AP2 and phosphorylates μ2. (A) The presence of either GAK/auxilin-2 or AAK1 was analyzed throughout the purification procedure of AP2. Both kinases were detectable in crude CCVs. However, GAK was depleted during subsequent enrichment of AP2, whereas AAK1 copurified with AP2 to a significant degree (HAP, pools 3 and 4). (B) Incubation of pools 3 and 4, obtained after HAP chromatography (Fig. 1 A), revealed a much higher kinase activity toward μ2 in pool 4 than in pool 3, corresponding to the enrichment of AAK1 in pool 4.