Table I. Evidence for the role of NFAT isoforms in the development of numerous cell types.

Tissue/cell type	NFAT isoform implicated	Methods used to determine role for NFAT	Target gene	Function	References
Heart	NFATC1	NFATC1−/− mice	?	Development of heart valve	de la Pompa et al., 1998; Ranger et al., 1998
Vascular endothelial cells (primary cells)	NFATC2	EMSA and reporter assays1; PG rescue of CSA effect in vitro/in vivo	COX2	VEGF signaling; PG synthesis during angiogenesis	Armesilla et al., 1999; Hernandez et al., 2001
Perivascular tissue	NFATC3/C4	NFATC3/C4 double null mice	?	Organization of developing blood vessels	Graef et al., 2001a
Skeletal muscle reserve cells	NFATC1	Overexpression and inhibition of NFAT in primary muscle cultures	myf-5	Expression of myf5	Friday and Pavlath, 2001
Skeletal muscle myotubes	NFATC2	NFATC2−/− mice	?	Skeletal muscle growth	Horsley et al., 2001
Skeletal muscle myoblasts	NFATC3	NFATC3−/− mice	?	Formation of primary myofibers	Kegley et al., 2001
Chondrocytes	NFATC2	NFATC2−/− mice	?	Inhibition of chondrogenesis	Ranger et al., 2000
Keratinocytes (primary cells)	NFATC3	Reporter assays2; NFAT inhibition; CSA treatment	p21WAF1/CIP	Activation of p21 during differentiation	Santini et al., 2001
Adipocytes (cell line)	NFATC2/C4	EMSA and reporter assays3; CSA treatment	aP2	Expression of adipocyte specific genes during differentiation	Ho et al., 1998

PG, prostaglandin.

¹COX2 promoter construct.

²NFAT reporter and p21 promoter constructs.

³aP2 promoter construct.