Table II. Evidence for the role of NFAT in cellular adaptation.

Cell type	NFAT isoform implicated	Methods used to determine role for NFAT	Target gene	Function	References
Pancreatic islet cells (cell line)	NFATC2	EMSA and reporter assays1	Glucagon	Expression of glucagon	Furstenau et al., 1999
Epidermal cell (cell line)	?	Reporter assays2 in vitro  and in vivo	?	Response to UV radiation	Huang et al., 2000
Cardiac muscle	NFATC4	Overexpression in  transgenic mice	?	Cardiac hypertrophy	Molkentin et al., 1998
Skeletal myofibers (in vitro)	NFATC1	Nuclear localization	?	Response to nerve stimuli	Liu et al., 2001
Skeletal muscle cells (cell line)	?	Reporter assays3	MyHC II	Expression of MyHC II isoforms	Allen et al., 2001
VSMCs (primary cells)	NFATC1/C2	Reporter assays4	?	Agonist induced signaling	Boss et al., 1998
ISMCs (isolated tissue)	NFATC3	Nuclear localization;  reporter assays5	?	Response to PDGF	Stevenson et al., 2001

EMSA, electrophoretic mobility shift assay.

¹Glucagon promoter constructs.

²NFAT reporter construct; NFAT-luciferase transgenic mice.

³MyHC II promoter constructs.

⁴NFAT reporter construct.

⁵NFAT-luciferase transgenic mice.