Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Mar 4;156(5):817–828. doi: 10.1083/jcb.200112059

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Different subcellular distributions of 14-3-3 and its ligands. (A) U2OS cells immunostained for 14-3-3σ (left) and corresponding DAPI staining (right). Bar, 10 μm. (B) Biochemical fractionation of U2OS cells followed by immunoblotting using an antibody recognizing all 14-3-3 isotypes (N, nuclear fraction; C, cytoplasmic fraction). Blots were probed with anti–α-tubulin (cytoplasmic) or anti–lamin B (nuclear) as controls. A very small amount of 14-3-3 is present in the nuclear fraction but cannot be seen in this exposure. (C) U2OS cells were metabolically labeled with [³⁵S]methionine, fractionated, and nuclear (N) and cytoplasmic (C) fractions containing equal amounts of protein and radioactivity were incubated with beads containing GST–14-3-3 or GST alone. Bound proteins were visualized by SDS-PAGE and autoradiography. A small portion of the input sample is shown on the left (Lysate lanes). Positions of molecular weight markers, in kD, are indicated. The prominent band at ∼28 kD in the cytoplasmic/14-3-3–GST lane (arrow) is endogenous U2OS 14-3-3 (Rittinger et al., 1999).