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. 2002 Mar 4;156(5):817–828. doi: 10.1083/jcb.200112059

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — LMB sequesters endogenous 14-3-3 in the nucleus. (A) Immunostaining of U2OS cells for endogenous 14-3-3σ with (right) or without (left) treatment with LMB for 18 h. Corresponding DAPI staining is shown on the bottom. Bar, 10 μm. (B) Quantitative analysis of 14-3-3σ location. Subcellular localization of 14-3-3σ was scored according to whether it was higher in the nucleus (N > C), evenly distributed between nucleus and cytoplasm (C = N), or higher in cytoplasm (C < N) for 300 cells at each time point. Results are the mean ± SD from three separate experiments.