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. 2002 Mar 4;156(5):855–865. doi: 10.1083/jcb.200105055

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Regulation of microtubule-based motion. To investigate regulation of microtubule motors, their activity was studied in the absence of functional myosin V. Shown are (modified) averages of travel distances (as described in Materials and methods) and velocities of plus and minus end runs. A run was defined as a period of uninterrupted motion along the microtubule (as described in Materials and methods). (A) The change from dispersion to aggregation coincides with a change in the length of (long-type) minus end runs, whereas the length of plus end runs remains constant. (B) The average velocity of (short-type) plus and minus end runs. There is no significant change in run velocity in either direction due to the change from dispersion to aggregation. These data show an average of ∼400 individual runs from four or more cells. M-V, myosin V. The error bars are the standard error of the mean.