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. 2002 Mar 4;156(5):855–865. doi: 10.1083/jcb.200105055

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Copyright © 2002, The Rockefeller University Press

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Figure 8. — The ability of motors to interact with melanosomes and microtubules does not change between aggregation and dispersion. (A) Melanosomes were purified from cells treated with the aggregating stimulus, melatonin, or the dispersing stimulus, MSH, and Western blots were performed with antibodies against myosin V (M-V), kinesin II (K-II), and cytoplasmic dynein (Dy). Note that the amount of microtubule motors on melanosomes does not change due to aggregation (A) or dispersion (D), whereas the amount of myosin V is higher on melanosomes purified from cells dispersing the pigment than from cells aggregating the pigment. (B) Binding of kinesin II (K-II) or cytoplasmic dynein (Dy) to microtubules in the presence of AMP-PNP. Bovine brain microtubules were added to extracts from cells aggregating (A) or dispersing (D) pigment, the mixture was spun through a glycerol cushion, and the pellets were analyzed for the presence of motors by Western blotting (experimental protocol from Reese and Haimo (2000).