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. 2003 Mar 3;160(5):769–780. doi: 10.1083/jcb.200208043

Figure 7.

Figure 7.

BAPTA-AM inhibits α2β1-mediated platelet adhesion and spreading on surfaces coated with GFOGER peptide. (A, 1) Washed human platelets (5 × 108/ml) were loaded with 10 μM Fura2-AM for 1 h at 30°C and then washed and resuspended to 2 × 107/ml in modified Tyrodes buffer with the inhibitor cocktail. 2 × 106 platelets were added to a GFOGER-coated coverslip. Fluorescence imaging and Ca2+ concentration measurements were performed with fluorescent microscopy using Openlab software for Macintosh. Results are representative of three experiments. (A, 2) The calcium concentration in a single platelet is shown. (B, 1) Washed human platelets (2 × 107/ml) were pretreated with the inhibitor cocktail, with or without 10 μM BAPTA-AM for 20 min. Platelets were seeded on BSA- or GFOGER-coated coverslips and stained and photographed as described in Fig. 1. (B, 2) The data represents the mean number of adhered platelets ± SEM per 0.028 mm2 from five different images from three experiments. The mean ± SEM diameter of 200 platelets from three experiments was measured. **, P < 0.01.