Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Mar 3;160(5):753–767. doi: 10.1083/jcb.200212114

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — FAK activates Rac and serum stimulation promotes GFP-FAK localization to lamellipodia. (A) Rac activation was visualized in the indicated cells by pull-down assays using GST-PAK (67–150) followed by Rac blotting. Total Rac expression is shown in whole cell lysates. (B) FAK^−/− v-Src cells were infected with Ad-TA or the indicated Ad-FAK constructs, and Rac activation was evaluated by GST-PAK binding. (C) FAK^−/− v-Src cells were transfected with GFP-FAK, starved, and then stimulated with 10% FBS. Time-lapse confocal video-microscopy at 1-min intervals was obtained over 3 h (Video 1 available at http://www.jcb.org/cgi/content/full/jcb.200212114/DC1). Panels show a 9-min time series where GFP-FAK is stably localized to focal contacts and transiently recruited to lamellipodia extensions (arrows).

Figure 6. — FAK activates Rac and serum stimulation promotes GFP-FAK localization to lamellipodia. (A) Rac activation was visualized in the indicated cells by pull-down assays using GST-PAK (67–150) followed by Rac blotting. Total Rac expression is shown in whole cell lysates. (B) FAK^−/− v-Src cells were infected with Ad-TA or the indicated Ad-FAK constructs, and Rac activation was evaluated by GST-PAK binding. (C) FAK^−/− v-Src cells were transfected with GFP-FAK, starved, and then stimulated with 10% FBS. Time-lapse confocal video-microscopy at 1-min intervals was obtained over 3 h (Video 1 available at http://www.jcb.org/cgi/content/full/jcb.200212114/DC1). Panels show a 9-min time series where GFP-FAK is stably localized to focal contacts and transiently recruited to lamellipodia extensions (arrows).