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. 2002 Dec 9;159(5):807–819. doi: 10.1083/jcb.200205068

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — MAD1, MAD2, and nup-encoding genes of the Nup53p-containing complex interact genetically. (A) The growth of WT, mad1Δ, mad2Δ, nup170Δ, nup157Δ, nup53Δ, and nup59Δ strains and combinations of double mutants were assessed at 27, 37, and 30°C (not depicted). Strains were grown to early logarithmic phase in YEPD at 30°C, diluted, spotted on YEPD and YEPD containing 20 μg/ml benomyl (BEN), and incubated at the indicated temperatures (27 or 37°C). (B) Benomyl resistance of nup mutants. nup59Δ, pom152Δ, nup170Δ, nup188Δ, nup100Δ, and mad1Δ strains, using W303 as the parental WT strain (WT 303), were spotted on YEPD plates containing either 20 or 25 μg/ml of benomyl and incubated for 3 d at 27°C.