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. 2002 Dec 9;159(5):753–763. doi: 10.1083/jcb.200207115

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Visualizing the spatial organization of 11p15 relative to the 11p chromosome territory. FISH of selected probes from 11p15.3, 11p15.4, or 11p15.5 (red) together with an HSA11p paint (green) to MAA-fixed lymphoblast (a) or primary fibroblast (b) nuclei. Known genes are italicized. (c) Maximal intensity projection of image stacks after three-dimensional FISH with probes from 11p15.5 (red) and a paint for 11p (green) on pFa-fixed fibroblast nuclei. Cells were counterstained with DAPI (blue). (d and e) Views of MAPaint reconstructions of pFa fixed fibroblast nuclei after three-dimensional FISH with probes (red) for IGF2 (d) and 80N22 (e) together with paints (green) for 11p (d) and 11q (e). Bars, 5 μm.