Figure 9.
Recruitment of the Arp2/3 complex to vinculin is not required for cell adhesion or cell migration. Vinculin-null cells reexpressing vector alone (Vin−/−), WT vinculin, or mutant vinculin (P878A) were examined for the ability to adhere to 50 μg/ml FN (A) or migrate through Transwell filters (B). The mean number of cells expressing vector alone that migrated through the filter was set to 100%. The relative number of cells/field ± SEM was calculated from the means of three independent experiments.