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. 2002 Dec 9;159(5):845–854. doi: 10.1083/jcb.200208018

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Genetic background of complementation lines. (A) PCR analysis of heterozygous (ppi2/TOC159) and homozygous (ppi2/ppi2) ppi2 plants expressing atToc159mGTP (159mGTP; line B0502) and homozygous ppi2 plants expressing full-length wild-type atToc159 (159wt, line B0205). The first reaction indicates the presence of undisrupted TOC159, the second the presence of the respective transgene, and the third indicates the presence of the 3′ end of TOC159 (control reaction). (B) Expression analysis of atToc159 (159wt) and atToc159mGTP (159mGTP) in a homozygous ppi2 background using RT-PCR. (C) Control PCR on total RNA after DNase treatment, but without reverse transcription.