Table I. Chromosomal events in the stable cell lines with synthetic 11mer repeats.
| Synthetic repeats inserted in BAC (Input DNA) |
Experiments | Analyzed cell lines |
Chromosomal events of introduced DNAa | |
|---|---|---|---|---|
| De novo artificial chromosome |
Integration into host chromosome (centromeric heterochromatin/arm) |
|||
| Alphoid repeats with functional CENP-B boxes (60 kb) | 1st | 15 | 7 | 8 (7/1) |
| (pWTR11.32) | 2nd | 12 | 5 | 7 (6/1) |
| Alphoid repeats with modified CENP-B boxes (60 kb) | 1st | 20 | 0 | 20 (9/11) |
| (pMTR11.32) | 2nd | 18 | 0 | 18 (7/11) |
| RF322 repeats with functional CENP-B boxes (69 kb) | 1st | 14 | 0 | 14 (5/9) |
| (pRF322B.192) | 2nd | 21 | 0 | 21 (3/18) |
| RF322 repeats without CENP-B boxes (69 kb) | 1st | 11 | 0 | 11 (3/8) |
| (pRF322L.192) | 2nd | 11 | 0 | 11 (2/9) |
a
Chromosomal events were determined according to the predominant pattern (>50%) of introduced DNA containing synthetic α21-I 11mer alphoid repeat units or synthetic RF322 repeats, using FISH analysis of chromosome spreads. These patterns were counted in cases in which all three signals, DNA (DAPI), BAC, and α21-I alphoid signals, were colocalized. A χ2 test of the predominant chromosomal events between pWTR11.32 and other cell lines with introduced plasmids showed highly significant P values (<0.0002).