Table I. Effect of VASP on the motility behavior of beads functionalized with different surface densities of ActA.
|
|
Surface density |
Beads that form tails
|
Average time period before movement stops
|
|---|---|---|---|
| molecules/100 nm2 | % | min | |
| − VASP | 0.85 | 10 | 10–15 |
| + VASP | 0.85 | 97 | 60–90 |
| − VASP | 1.36 | 90 | 40 |
| + VASP | 1.36 | 98 | >120 |
| − VASP | 1.85 | 94 | 45 |
| + VASP | 1.85 | 100 | >120 |
| − VASP | 2.12 | 97 | 60–80 |
| + VASP | 2.12 | 100 | >120 |
Movement of beads was recorded until the actin tails detached from the beads. The time at which 50% of the beads (n = 200) had lost their tails was noted.