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. 2003 Oct 13;163(1):71–81. doi: 10.1083/jcb.200303003

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Primary structure of CIIA. (A) Predicted amino acid sequences of the mouse CIIA. The cDNA sequence data of the mouse CIIA are available from GenBank/EMBL/DDBJ under accession no. AF373710. (B) Distribution of the CIIA transcript in mouse tissues. A multiple-tissue Northern blot membrane was probed with ³²P-labeled BamHI–AccI fragment (714 bp) of mouse CIIA cDNA. (C) Immunodetection of CIIA protein in adult mouse brain tissue. 50 μg cell lysates were analyzed by immunoblot (IB) using a rabbit preimmune IgG or anti-CIIA antibody. The anti-CIIA antibody was produced from rabbits immunized with hexahistidine (His)-tagged CIIA and affinity-purified. Immunoblots with anti-HA antibody of the lysates from 293T cells transfected with pcDNA3-HA empty vector or pcDNA3-HA-CIIA were also shown.