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. 2002 Mar 18;156(6):1015–1028. doi: 10.1083/jcb.200201002

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Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 1. — Proteins with identity to Vac14p exist in higher eukaryotes. Identical amino acids (black) and similar amino acids (gray) are highlighted. (Left) The NH₂-terminal sequence of S. cerevisiae VAC14 and related ORFs were aligned using ClustalW (http://searchlauncher.bcm.tmc.edu:9331/multi-align/multi-align.html). Sequences were identified by searching the indicated databases via the BLAST algorithm (Altschul et al., 1990). The C. albicans sequence was found in the C. albicans database (http://sequence-www.stanford.edu/group/candida/search.html). ORFs from S. pombe (EMBL/GenBank/DDBJ accession no. CAB08779.1), A. thaliana (EMBL/GenBank/DDBJ accession no. AAD12702.1), C. elegans (EMBL/GenBank/DDBJ accession no. CAB00043.1) and D. melanogaster (EMBL/GenBank/DDBJ accession no. AAF54829.1) were in the GenBank database (http://www.ncbi.nlm.nih.gov/BLAST). The M. musculus sequence was identified in the mouse EST database (http://www.ncbi.nlm.nih.gov/BLAST). The sequence shown is a consensus of two similar ESTs (EMBL/GenBank/DDBJ accession nos. BE573148 and BF162275). The H. sapiens sequence was in the human EST database (http://www.ncbi.nlm.nih.gov/BLAST). The consensus sequence of 14 similar ESTs from chromosome 16 (EMBL/GenBank/DDBJ accession nos. AL527155, AL535971, AL555680, AL556062, BE409891, BE696780, BE728471, BE893810, BE901196, BE937614, BF081182, BF091052, BF325708, and BG107035) is shown. The sequences contain at least 25% global identity and 42% global similarity to S. cerevisiae VAC14. (Right) The COOH-terminal sequence of yeast Vac14p and similar ORFs were identified and aligned as in the left sequence. The M. musculus sequence is a consensus of 12 similar ESTs (EMBL/GenBank/DDBJ accession nos. AA036005, AA050423, AA058300, AA276168, AA497446, AA670618, BE862623, BF023070, BF237130, BF720417, BG079707, W09660). The H. sapiens sequence is hypothetical protein, FLJ10305 found on chromosome 16, deposited in the human genome database (http://www.ncbi.nlm.nih.gov/genome/seq). One of the mouse ESTs (clone ID 468926) had been mapped to chromosome VIII (106 cM offset) with an inferred position on human chromosome 16 (16q22.1-qter).

Figure 1. — Proteins with identity to Vac14p exist in higher eukaryotes. Identical amino acids (black) and similar amino acids (gray) are highlighted. (Left) The NH₂-terminal sequence of S. cerevisiae VAC14 and related ORFs were aligned using ClustalW (http://searchlauncher.bcm.tmc.edu:9331/multi-align/multi-align.html). Sequences were identified by searching the indicated databases via the BLAST algorithm (Altschul et al., 1990). The C. albicans sequence was found in the C. albicans database (http://sequence-www.stanford.edu/group/candida/search.html). ORFs from S. pombe (EMBL/GenBank/DDBJ accession no. CAB08779.1), A. thaliana (EMBL/GenBank/DDBJ accession no. AAD12702.1), C. elegans (EMBL/GenBank/DDBJ accession no. CAB00043.1) and D. melanogaster (EMBL/GenBank/DDBJ accession no. AAF54829.1) were in the GenBank database (http://www.ncbi.nlm.nih.gov/BLAST). The M. musculus sequence was identified in the mouse EST database (http://www.ncbi.nlm.nih.gov/BLAST). The sequence shown is a consensus of two similar ESTs (EMBL/GenBank/DDBJ accession nos. BE573148 and BF162275). The H. sapiens sequence was in the human EST database (http://www.ncbi.nlm.nih.gov/BLAST). The consensus sequence of 14 similar ESTs from chromosome 16 (EMBL/GenBank/DDBJ accession nos. AL527155, AL535971, AL555680, AL556062, BE409891, BE696780, BE728471, BE893810, BE901196, BE937614, BF081182, BF091052, BF325708, and BG107035) is shown. The sequences contain at least 25% global identity and 42% global similarity to S. cerevisiae VAC14. (Right) The COOH-terminal sequence of yeast Vac14p and similar ORFs were identified and aligned as in the left sequence. The M. musculus sequence is a consensus of 12 similar ESTs (EMBL/GenBank/DDBJ accession nos. AA036005, AA050423, AA058300, AA276168, AA497446, AA670618, BE862623, BF023070, BF237130, BF720417, BG079707, W09660). The H. sapiens sequence is hypothetical protein, FLJ10305 found on chromosome 16, deposited in the human genome database (http://www.ncbi.nlm.nih.gov/genome/seq). One of the mouse ESTs (clone ID 468926) had been mapped to chromosome VIII (106 cM offset) with an inferred position on human chromosome 16 (16q22.1-qter).