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. 2002 Mar 18;156(6):1015–1028. doi: 10.1083/jcb.200201002

Figure 8.

Figure 8.

PtdIns(3,5)P 2 is required for osmotic stress induced fragmentation of the vacuole. Wild-type (a and e), vac141 (b and f), fab11 (c and g), vac71 (d and h) cells were labeled with FM4-64 to visualize vacuole membranes. Cells were incubated in YEPD alone (a–d) or YEPD containing 0.4 M NaCl (e–h) for 10 min. Bar, 8 μm.