Table III.
Vac14p is required for normal elevation of PtdIns(3,5)P 2 in response to osmotic stress
| PtdIns(3)P | PtdIns(3,5)P2 | |||
|---|---|---|---|---|
| + | − | + | − | |
| vac14-Δ1 with pRS426 | 112 ± 10 | 100 ± 2 | 2.4 ± 0.3 | ≤1.5 |
| vac14-Δ1 with FAB1 CEN | 100 ± 8 | 92 ± 16 | 3.2 ± 0.8 | 2.0 ± 0.3 |
| vac14-Δ1 with FAB1 2μ | 84 ± 8 | 84 ± 8 | 3.1 ± 0.4 | 2.1 ± 0.8 |
| vac14-Δ1 with VAC14 CEN | 55 ± 3 | 76 ± 3 | 28 ± 12 | 3.2 ± 0.8 |
| vac14-Δ1 with VAC14 2μ | 60 ± 16 | 80 ± 24 | 33 ± 0.4 | 3.2 ± 0.8 |
Cells were grown in inositol free media in the presence of 0.01 mCi/ml 3H inositol. 0.9 M NaCl was added to half the cultures for 10 min before extraction. The values were generated as described in Table II and normalized to wild-type (Table II). The data (mean ± standard deviation) were calculated from results obtained in four independent experiments.