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. 2002 Mar 18;156(6):1003–1013. doi: 10.1083/jcb.200111093

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — KNF encodes α-glucosidase I. (A) The ER resident glycoprotein, PDI, shows reduced mobility in extracts from knf embryos. 10 μg of total protein was probed with PDI antisera. Left, molecular mass standards. (B) knf embryo extracts lack α-glucosidase I activity. Wt and knf embryo extracts were incubated for 24 h with the rhodamine-conjugated triglucoside substrate G_α2G_α3G_α-TMR to test for α-glucosidase I activity, or with the rhodamine-conjugated diglucoside substrate G_α3G_α-TMR to test for glucosidase II activity. Reaction products were separated and measured by capillary electrophoresis laser-induced fluorescence detection at 580 nm. Left column, α-glucosidase I activity; right column, α-glucosidase II activity; y-axis, signal (V); x-axis, migration time (s).